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mitotracker cmxred  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mitotracker cmxred
    STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by <t>MitoTracker</t> CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.
    Mitotracker Cmxred, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitotracker cmxred/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mitotracker cmxred - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "STAT3 Regulates Mouse Neural Progenitor Proliferation and Differentiation by Promoting Mitochondrial Metabolism"

    Article Title: STAT3 Regulates Mouse Neural Progenitor Proliferation and Differentiation by Promoting Mitochondrial Metabolism

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2020.00362

    STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by MitoTracker CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.
    Figure Legend Snippet: STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by MitoTracker CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.

    Techniques Used: Activity Assay, Membrane, Staining, Flow Cytometry, Western Blot, Activation Assay



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    mitotracker cmxred  (Thermo Fisher)
    90
    Thermo Fisher mitotracker cmxred
    STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by <t>MitoTracker</t> CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.
    Mitotracker Cmxred, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitotracker cmxred/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mitotracker cmxred - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by MitoTracker CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: STAT3 Regulates Mouse Neural Progenitor Proliferation and Differentiation by Promoting Mitochondrial Metabolism

    doi: 10.3389/fcell.2020.00362

    Figure Lengend Snippet: STAT3 regulates mitochondrial respiration and ROS production in neural progenitors. (A–E) OCR of E14 neural progenitors was comparable between CTL and Stat3 cKO. But at E17, the OCR of Stat3 cKO was significantly decreased compared to the CTL. (F) The Complex I activity was compromised in E17 Stat3 cKO neural progenitors. (G,H) E17 Stat3 cKO neural progenitors had lower mitochondrial membrane potential indicated by MitoTracker CMXROS staining. (I) qPCR showed that the ratio of mitochondrial-DNA to nuclear-DNA is not significantly changed between CTL and Stat3 cKO neural progenitors. (J) ADP/ATP ratio assay showed that Stat3 cKO neural progenitors had elevated ADP/ATP ratio. (K,L) Flow cytometry showed that Stat3 cKO neural progenitors had increased mitochondrial superoxide production. (M,N) Flow cytometry showed that Stat3 cKO neural progenitors contained increased cellular ROS. (O) Western blot showed that Stat3 cKO neural progenitors had elevated AMPK pathway activation accompanied by decreased mTOR. (P) Stat3 cKO resulted in increased activation of MAPK14 and AKT, as well as increased CASP3 cleavage. Each lane was sample collected from individual embryo of the same little. Oligo, oligomycin; Rot, Rotenone; AA, antimycin A. Error bars represent mean ± standard deviations. * p < 0.05, ** p < 0.01, *** p < 0.005, n.s. not significant.

    Article Snippet: Neurospheres cultured for 4 days were dissociated into single cells with Accutase (Thermo Fisher Scientific) and was resuspended in SFM containing 5 μM Mitotracker CMXRed (Thermo Fisher Scientific), or 5 μM Mitosox Red (Thermo Fisher Scientific) or 5 μM CM-DCFDA (Thermo Fisher Scientific), respectively, and incubated in 37°C CO 2 incubator for 30 min.

    Techniques: Activity Assay, Membrane, Staining, Flow Cytometry, Western Blot, Activation Assay